The unmanned Cygnus cargo craft was packed with trash and sent on a fiery destructive reentry through the atmosphere.
Commander Scott Kelly and Timothy Kopra sent computer commands to set the Cygnus free.
Kelly, who will soon end a yearlong mission aboard the ISS, radioed thanks to the ground for the supplies.
“It’s been a pleasure,” Scott Kelly said as the capsule slowly backed away from the Space Station, its two circular solar wings looking like open umbrellas.
Carrying more than a ton of garbage, the Cygnus is scheduled to burn up in the atmosphere on Saturday.
Virginia-based Orbital ATK plans to launch another Cygnus with more supplies from Cape Canaveral, Florida, late next month.